Description
OVERVIEW
PRODUCT SPECIFICATIONS Substrates, Support Films for Transmission Electron Microscopy Grids PELCO® TEM grid Support Films of Formvar, Formvar/Carbon, Carbon, and Silicon Monoxide are available on the following 3.05mm O.D. grids: 0.4 x 2mm single slot Cu, 75 mesh Cu, 200 mesh Cu,Ni or Au, 200 mesh Hex Mo, 300 mesh Cu, Ni or Au, 300 mesh Hex Mo, and 400 mesh Cu, Ni, Au, Al, Ti and stainless steel. Support films on the finer mesh grids can withstand considerable handling during specimen preparation. Those on slot and 75 mesh grids require more gentle handling and are ideal for those applications requiring large viewing areas without grid bar interference. Our popular line of PELCO NetMesh™ Grids (our trademarked lacey films) are available on 200, 300 and 400 mesh grids. These robust films allow for viewing of specimens without interference from underlying support film material. The complete PELCO® line of support films meets the requirements of most applications in all fields of electron microscopy. TEM Support Film Product Note, How Material is Layered on the Grid. Consult the Support Film / Substrate Application Guide below for suggestions on support film choice: PELCO® TEM Grid Support Film Application Guide This Support Film Application Guide will help you determine what support film to use for your particular transmission electron microscopy project. Once you have decided which substrate best suits your needs, you can go back up on the page for a listing of available Support Films and pricing. B = Best; G = Good Alternative; – = Not Suitable Substrate Application Formvar Only
Formvar Stabilized with Carbon
Silicon Monoxide on Formvar
Silicon Monoxide on Type-A
Carbon Type-A
Carbon Type-B
Pure Carbon Film
Is Lacey Film suitable for this application? Applications Requiring Pure Formvar
B
–
–
–
–
–
–
No Bacterial Suspensions
–
G
B
B
B
B
B
Yes Cell Fragment Suspensions
–
B
B
B
B
B
B
Yes Diffraction Studies
–
–
G
G
B
B
B
Yes EDS (energy dispersive spectrometry)
–
G
–
–
B
B
G
Yes High Resolution Microscopy
–
G
G
B
B
B
B
Yes High Temperature Techniques / Heating Stage
–
–
–
G
B
–
B Yes (Type A) Low Magnification Microscopy
G
B
B
G
G
B
G
No Particulate Suspension, Biological
–
G
B
B
B
B
B
Yes Particulate Suspension, Non-biological
–
G
B
B
B
B
B
Yes Powders, Dry
–
G
B
G
G
B
G
No Replicas & Low Temperature Techniques
G
B
–
–
G
B
G Yes (Type-A) Thin Sections
G
B
G
B
G
B
G
Yes Viral Suspensions
–
G
G
B
B
B
B
Yes Typically packaged in PELCO® TEM Grid Storage Box (Product Number 160) but is subject to change to other equally suitable grid storage boxes. Carbon Type-A Carbon support films with a removable Formvar on the opposite side of the grid. When the Formvar is removed, by dipping in solvent, a pure carbon film remains. (Note: The Ultrathin Carbon Film on a Lacey Carbon Support Film, Prod. No. 01824, has no Formvar backing.) These films are stable under all EM operating conditions and are for use where the presence of a Formvar layer cannot be tolerated. Pure carbon films tend to be more delicate than those with a Formvar backing and require more delicate handling during specimen preparation than most other support films. Ultrathin Carbon Film on a Lacey Carbon Support Film: Pure carbon support films with no Formvar backing. This new product has an even thinner carbon film which is mounted on a Lacey carbon film. The carbon support layer lying over the holes is less than 3nm in thickness and is the thinnest support film available. It is particularly useful for high resolution microscopy of low contrast particles and is also ideal for use with the Energy Filtering TEM. 01824G – Ultrathin Carbon Film on Lacey Carbon Support Film, 300 mesh, Gold – pkg/25 01824 – Ultrathin Carbon Film on Lacey Carbon Support Film, 400 mesh, Copper – pkg/25 PELCO® Technical Note, Ultrathin Carbon Film Supported by a Lacey Carbon Film on a 400 Mesh Copper Grid (77KB PDF) PELCO® Technical Note, Ultrathin Carbon Film Supported by a Lacey Carbon Film on a 300 Mesh Gold Grid (153KB PDF)
